The Complete Amino-Acid Sequence of Hen Ovalbumin
نویسندگان
چکیده
منابع مشابه
The complete amino-acid sequence of hen ovalbumin.
The complete amino acid sequence of hen ovalbumin, comprising 385 residues, has been determined. The sequence was deduced from the 17 cyanogen bromide fragments and from peptides derived by digestion with a number of proteolytic enzymes. The molecular weight of the polypeptide chain of ovalbumin is 42699. Ovalbumin has four sites of postsynthetic modification; in addition to the acetylated N te...
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The active cysteine of papain was labeled with W-iodoacetate and the cystine residues were reduced and coupled with unlabeled iodoacetate. The heptacarboxymethyl papain was then maleylated and hydrolyzed with trypsin. Key peptides were isolated from this hydrolysate which have permitted completion of the amino acid sequence of the protein. Thus, an earlier tentative and incomplete version of th...
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Cyanogen bromide treatment of reduced, S-carboxymethylated phosphoglycerate kinase yielded 14 major peptides, CNBr-1 (20 residues), CNBr-2 (8 residues), CNBr-3 (33 residues), CNBr-4 (11 residues), CNBr-5 (104 residues), CNBr-6 (14 residues), CNBr-7 (37 residues), CNBr-8 (7 residues), CNBr-9 (6 residues), CNBr-10 (11 residues), CNBr-11 (19 residues), CNBr-12 (42 residues), CNBr-13 (44 residues),...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1981
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1981.tb05243.x